《植物生理学报》 2017, 53(3): 308-316

Mapping-by-sequencing在作物农艺性状相关基因定位的应用

杨琳, 杨剑飞, 王欢欢, 王宇^*, 李玉花^*

东北林业大学生命科学学院, 哈尔滨150040

通信作者：王；E-mail: vonov@sina.com; lyhshen@126.com

摘　要：

通过挖掘控制重要农艺性状的基因以提高农作物产量是育种学家一直关注的问题。然而由于农作物遗传背景复杂且基因组庞大, 该过程往往步骤繁琐, 耗时长且成本较高。Mapping-by-sequencing (利用高通量测序进行基因定位, MBS)利用二代测序技术和遗传分离群体混池建库分析的方法成功解决了上述问题, 加快了农作物重要农艺性状的基因定位同时也降低了研究成本。该方法已经成功应用于重要农艺性状相关联的突变位点鉴别和基因定位。本文综述了Mapping-by-sequencing的实验原理, 并总结了该方法在水稻、大麦、小麦及玉米等农作物中与目标农艺性状相关基因定位的应用。

关键词：Mapping-by-sequencing; Bulked segregant analysis; 基因定位; 作物

收稿：2016-11-21   修定：2017-02-22

资助：国家自然科学基金(31471911、31272200和31401907)和黑龙江省自然科学基金(C2015052)。

The application of Mapping-by-sequencing applies in crops

YANG Lin, YANG Jian-Fei, WANG Huan-Huan, WANG Yu^*, LI Yu-Hua^*

College of Life Science, Northeast Forestry University, Harbin 150040, China

Corresponding author: WANG Yu; E-mail: vonov@sina.com; lyhshen@126.com

Abstract:

In order to design better crops, forward genetic approach was usually used to screen loss-of-function genes related to important agronomic traits. With the repetitive DNA, multiple gene copies, chromosomal rearrangements and large complex polyploidy crop genomes can considerably complicate to discover mutated gene by conventional forward genetics approaches. With the next-generation sequencing platforms, MBS technology (Mapping-by-sequencing) is a powerful tools for rapid identification of causal mutations in plants. MBS technology achieves this by sequencing genomic DNA of plants pooled from an F2 population based on Bulked segregant analysis (BSA). In this review we summarized the principle of Mapping-by-sequencing and the applications in rice, barley, wheat and maize for gene screening.

Key words: Mapping-by-sequencing (MBS); Bulked segregant analysis (BSA); genetic mapping; crops